Difference between revisions of "Part:BBa K3286205"

Revision as of 08:05, 21 October 2019

Vc2 Riboswitch + sfGFP

This composite part contains the Vc2 riboswitch (BBa_K3286202)[1], which was used in combination with composite part BBa_K3286204. The function of this part is that upon DSF sensing from part BBa_K3286204, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 riboswitch[2], which will result in sfGFP (BBa_K3286203) translation.

Figure 1: Comparison of a low copy number(left) and a high copy number (right) plasmid containing this BioBrick. Both are E. coli Dh5-Alpha cells incubated on a LB agar plate.

B. R. Pursley, M. M. Maiden, M. L. Hsieh, N. L. Fernandez, G. B. Severin, and C. M. Waters, “Cyclic di-GMP regulates TfoY in Vibrio cholerae to control motility by both transcriptional and posttranscriptional mechanisms,” J. Bacteriol., vol. 200, no. 7, pp. 1–19, 2018.
S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 392
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This  composite part contains the Vc2 riboswitch (<partinfo>BBa_K3286202</partinfo>)[1], which was used in combination with composite part <partinfo>BBa_K3286204</partinfo>. The function of this part is that upon DSF sensing from part <partinfo>BBa_K3286204</partinfo>, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 riboswitch[2], which will result in sfGFP (<partinfo>K3286203</partinfo>) translation.
-[[File:T--Wageningen UR--Constructs Edit BBa K3286205.jpg|750px|thumb|center|<b>Figure 1:</b> 1]]
+[[File:T--Wageningen UR--Constructs Edit BBa K3286205.jpg|750px|thumb|center|<b>Figure 1:</b> Comparison of a low copy number(left) and a high copy number (right) plasmid containing this BioBrick. Both are <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate.]]
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