Difference between revisions of "Part:BBa K3113006"
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<h2>Usage</h2>
 
<h2>Usage</h2>
  
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The informational readout of ALiVE is RNA. To load a specific RNA into the vesicles we fused it to RNA-motifs which are bound specifically by RNA binding proteins. Two combinations of binding protein and motif were tested. One was MCP, the MS2 bacteriophage coat protein, which binds to the MS2 RNA motif.
  
 
<h2>Biology</h2>
 
<h2>Biology</h2>
  
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The RNA hairpin motif that is recognized by the MS2 capsid protein dimers.<ref>Bleckley, Samuel, and Susan J Schroeder. “Incorporating global features of RNA motifs in predictions for an ensemble of secondary structures for encapsidated MS2 bacteriophage RNA.” RNA (New York, N.Y.) vol. 18,7 (2012): 1309-18. doi:10.1261/rna.032326.112</ref>
  
  

Revision as of 07:48, 21 October 2019


MS2-Stemloop

MS2 is a stemloop structure from the MS2 bacteriophage genome. This stemloop is used as an adapter for RNA binding and loading said RNA into extracellular vesicles

Usage

The informational readout of ALiVE is RNA. To load a specific RNA into the vesicles we fused it to RNA-motifs which are bound specifically by RNA binding proteins. Two combinations of binding protein and motif were tested. One was MCP, the MS2 bacteriophage coat protein, which binds to the MS2 RNA motif.

Biology

The RNA hairpin motif that is recognized by the MS2 capsid protein dimers.[1]


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Bleckley, Samuel, and Susan J Schroeder. “Incorporating global features of RNA motifs in predictions for an ensemble of secondary structures for encapsidated MS2 bacteriophage RNA.” RNA (New York, N.Y.) vol. 18,7 (2012): 1309-18. doi:10.1261/rna.032326.112