Difference between revisions of "Part:BBa K3137006"
Line 3: Line 3:
 
<partinfo>BBa_K3137006 short</partinfo>
 
<partinfo>BBa_K3137006 short</partinfo>
  
−
AbpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.
+
<i>abp</i>AB are two genes of the <i>E.coli</i> genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained <i>abp</i>A and <i>abp</i>B by PCR from the genome of <i>E.coli</i> BL21 and constructed recombinant plasmid that connected <i>abp</i>A and <i>abp</i>B at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.
 +
 
 +
===Usage and Biology===
 +
 
 +
<i>abp</i>AB encodes the protein AbpAB, which can inhibit the replication and late gene expression of phage, resulting in blocking of phage propagation. AbpAB has no effect on the bacterial growth(Fig 1) which is important to the industry. However, protein AbpAB didn’t work well as we expected(Fig 2).
 +
 
 +
[[Image:AbpAB 1.png|500px|center|thumb|Figure 1. The effect of protein AbpAB on growth of <I>E. coli</i> BL21]]
 +
 
 +
[[Image:AbpAB 2.png|500px|center|thumb|Figure 2. <i>E. coli</i> BL21-pET28a（left）and <i>E. coli</i> BL21-pET28a-abpAB]]
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:41, 21 October 2019


abpAB

abpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.

Usage and Biology

abpAB encodes the protein AbpAB, which can inhibit the replication and late gene expression of phage, resulting in blocking of phage propagation. AbpAB has no effect on the bacterial growth(Fig 1) which is important to the industry. However, protein AbpAB didn’t work well as we expected(Fig 2).

Figure 1. The effect of protein AbpAB on growth of E. coli BL21
Figure 2. E. coli BL21-pET28a（left）and E. coli BL21-pET28a-abpAB


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal BglII site found at 561
    Illegal BglII site found at 1621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
    Illegal NgoMIV site found at 3333
  • 1000
    COMPATIBLE WITH RFC[1000]