Difference between revisions of "Part:BBa K149001:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K149001 short</partinfo>
 
<partinfo>BBa_K149001 short</partinfo>
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===Design Notes===
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===Characterization===
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None
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===Source===
 
===Source===
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The PRP22 promoter sequence was originally extracted from S. Cervisiae, using the 2 following primers:
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Simon St. Pierre extracted from S. Cervisiae genome.  
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TAGTAGGATCCATTATTCTGGGCATCCGT;ATACTGAATTCCTCTAATATCTTTGTGTTACCTATGT.  A BAMHI restriction site was included within the forward primer, while an EcoRV site was included within the reverse primer.  This work was completed by our laboratory technician Simon St-Pierre.  
  
 
===References===
 
===References===

Revision as of 20:26, 24 October 2008

Prp22 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Source

The PRP22 promoter sequence was originally extracted from S. Cervisiae, using the 2 following primers: TAGTAGGATCCATTATTCTGGGCATCCGT;ATACTGAATTCCTCTAATATCTTTGTGTTACCTATGT. A BAMHI restriction site was included within the forward primer, while an EcoRV site was included within the reverse primer. This work was completed by our laboratory technician Simon St-Pierre.

References