Difference between revisions of "Part:BBa K3113100"
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Revision as of 07:02, 21 October 2019
Gag HIV-1
This sequence codes for the coat protein of the human immunodeficiency virus, which mediates the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles.
Usage
For ALiVE, two vesicles were tested. One kind of vesicle is based on the Gag-p24 capsid protein of HIV. We used this protein fused together with RNA binding proteins to build and load virus-like vesicles for non-invasive cell monitoring.
Biologie
Gag-p24 is the group-specific antigen from the lentivirus HIV. It assembles at the plasma membrane and leads to the budding of vesicles.[1]
Characterization
Protein Structure
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1307
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 607
References
- ↑ HIV-1 Gag: a Molecular Machine Driving Viral Particle Assembly and Release Heinrich G. Göttlinger Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, and Department of Pathology, Harvard Medical School, Boston