Difference between revisions of "Part:BBa K103004"
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<partinfo>BBa_K103004 short</partinfo> | <partinfo>BBa_K103004 short</partinfo> | ||
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| + | [[Image:Z_spa.jpg]] | ||
Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003). | Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003). | ||
*Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) | *Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) | ||
| − | *We have used Z<sub>SPA-1</sub> protein fusions in our 'hunter and prey' selection system | + | *We have used Z<sub>SPA-1</sub> protein fusions with [[Part:BBa_K103019 | BBa_K103019]] or [[Part:BBa_K103020 | BBa_K103020]] as a prey in our 'hunter and prey' selection system |
| − | + | *This part uses our NdeI/SacI/BamHI fusion cloning substandard | |
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Revision as of 17:21, 24 October 2008
protein ZSPA-1
Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003).
- ZSPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
- We have used ZSPA-1 protein fusions with BBa_K103019 or BBa_K103020 as a prey in our 'hunter and prey' selection system
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]