Difference between revisions of "Part:BBa K103003"

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[[Image:Protein_a.jpg]]
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Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]
 
===Usage and Biology===
 
===Usage and Biology===
 
*Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
 
*Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
*We have exploited it's ability of binding Z<sub>SPA-1</sub> protein to create our 'hunter and prey system'
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*We have used this protein as hunter fused with [[Part:BBa_K103017 | BBa_K103017]] or [[Part:BBa_K103018 | BBa_K103018]] in our 'hunter and prey' selection system
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Revision as of 17:13, 24 October 2008

B domain of Staphylococcal protein A

B domain of Staphylococcal protein A, which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of ZSPA-1.

Protein a.jpg

Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]

Usage and Biology

  • Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
  • We have used this protein as hunter fused with BBa_K103017 or BBa_K103018 in our 'hunter and prey' selection system


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 91
    Illegal BamHI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]