Difference between revisions of "Part:BBa K3059618"
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<partinfo>BBa_K3059618 short</partinfo>
 
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This is SaSuhB. It forms macroscopic amalyoid fibers.  
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SaSuhB is a protein native to Staphylococcus aureus. While it is characterized as an inositol monophosphatase enzyme, it is also necessary for unimpaired pia-independent biofilm formation in S. aureus [1]. Moreover, further study of this protein has revealed that it is capable of forming macroscopic amyloid fibers when expressed in E. coli [2]. The fibers were characterized as being incredibly sticky, causing the researchers great trouble in trying to wash the fibers off of glass and plastic ware [2]. Additionally, SEM imagery revealed that bacteria were readily adhered to these fibers [2]. This combination of traits makes SaSuhB appear perfect for our application of forming strong, robust biofilms.  
  
 
<html><img src="https://2019.igem.org/wiki/images/4/45/T--William_and_Mary--SaSuhB_033.png" width="750px"/></html>
 
<html><img src="https://2019.igem.org/wiki/images/4/45/T--William_and_Mary--SaSuhB_033.png" width="750px"/></html>
  
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== References ==
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3059618 SequenceAndFeatures</partinfo>
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[1] Boles, B. R., Thoendel, M., Roth, A. J., & Horswill, A. R. (2010). Identification of genes involved in polysaccharide-independent Staphylococcus aureus biofilm formation. PloS one, 5(4), e10146.
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[2] Dutta, A., Bhattacharyya, S., Kundu, A., Dutta, D., & Das, A. K. (2016). Macroscopic amyloid fiber formation by staphylococcal biofilm associated SuhB protein. Biophysical chemistry, 217, 32-41.
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===Functional Parameters===
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<partinfo>BBa_K3059618 parameters</partinfo>
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Revision as of 06:44, 21 October 2019


Staphylococcus Aureus SuhB Protein (SaSuhB)

SaSuhB is a protein native to Staphylococcus aureus. While it is characterized as an inositol monophosphatase enzyme, it is also necessary for unimpaired pia-independent biofilm formation in S. aureus [1]. Moreover, further study of this protein has revealed that it is capable of forming macroscopic amyloid fibers when expressed in E. coli [2]. The fibers were characterized as being incredibly sticky, causing the researchers great trouble in trying to wash the fibers off of glass and plastic ware [2]. Additionally, SEM imagery revealed that bacteria were readily adhered to these fibers [2]. This combination of traits makes SaSuhB appear perfect for our application of forming strong, robust biofilms.

References

[1] Boles, B. R., Thoendel, M., Roth, A. J., & Horswill, A. R. (2010). Identification of genes involved in polysaccharide-independent Staphylococcus aureus biofilm formation. PloS one, 5(4), e10146. [2] Dutta, A., Bhattacharyya, S., Kundu, A., Dutta, D., & Das, A. K. (2016). Macroscopic amyloid fiber formation by staphylococcal biofilm associated SuhB protein. Biophysical chemistry, 217, 32-41.