Difference between revisions of "Part:BBa K3145001:Design"

Revision as of 06:03, 21 October 2019

sfGFP (Super-Folder Green Fluorescent Protein)

Assembly Compatibility:

There is C-terminal strep tag

The sfGFP sequence originated from our original pY71-sfGFP construct which was made using the Kwon & Jewett 2015 journal article in our references.

Kwon, Y.-C. & Jewett, M.C.High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Sci. Rep. 5,8663; DOI:10.1038/srep08663 (2015).
Bundy, B. C., and Swartz, J. R.Site-Specific Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation. Bioconjugate Chem.21,2,255-263;DOI: 10.1021/bc9002844

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 ===References===
 *<p align="justify"> Kwon, Y.-C. & Jewett, M.C.High-throughput preparation methods of crude extract for robust cell-free protein synthesis. [https://www.nature.com/articles/srep08663|<i>Sci. Rep.</I>] 5,8663; DOI:10.1038/srep08663 (2015).</p>
-*<p align="justify"> Bundy, B. C., and Swartz, J. R.Site-Specific Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation. [https://pubs.acs.org/doi/10.1021/bc9002844#|<i>Bioconjugate
+*<p align="justify"> Bundy, B. C., and Swartz, J. R.Site-Specific Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation. [https://pubs.acs.org/doi/10.1021/bc9002844#|<i>Bioconjugate Chem.</i>]21,2,255-263;DOI: 10.1021/bc9002844
-Chem.</i>21 (2), 255-263;DOI: 10.1021/bc9002844