Difference between revisions of "Part:BBa K3031015"

Revision as of 05:32, 21 October 2019

LuxI with FUR box located in the -10 region

This is the LuxI promoter sequence with a standard Ferric Uptake Regulator sequence (FUR box) inserted between the -35 and -10 region. The FUR box allows for the repression of Quroum Sensing expression of genes associated in the presence of ferric iron. In the classical FUR repression mechanism, iron-bound Fur binds to a Fur box sequence that overlaps with, or is proximal to, promoters of iron responsive genes, thus preventing their transcription. When intracellular iron is depleted, Fe2+ is released from Fur, causing conformational changes in the protein resulting in dissociation from the Fur box.

In a low iron environment, through the regulation of the LuxI-Fur box promoter, the LuxI protein (AHL) would be produced as normal which forms an integral part of the well characterized and used LuxR/LuxI quorum sensing system.

In the absence of Fe the downstream genes are once gain turned on at high cell densities.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3031015 short</partinfo>
-This is the LuxI promoter with a standard Ferric Uptake Regulator sequence inserted into the -10 region.
+This is the LuxI promoter sequence with a standard Ferric Uptake Regulator sequence (FUR box) inserted between the -35 and -10 region.
-The FUR box allows for the halting of QS expression of genes in the presence of ferric iron. In the absence of Fe the downstream genes are once gain turned on at high cell densities.
+The FUR box allows for the repression of Quroum Sensing expression of genes associated in the presence of ferric iron. In the classical FUR repression mechanism, iron-bound Fur binds to a Fur box sequence that overlaps with, or is proximal to, promoters of iron responsive genes, thus preventing their transcription. When intracellular iron is depleted, Fe2+ is released from Fur, causing conformational changes in the protein resulting in dissociation from the Fur box.
+In a low iron environment, through the regulation of the LuxI-Fur box promoter, the LuxI protein (AHL) would be produced as normal which forms an integral part of the well characterized and used LuxR/LuxI quorum sensing system.
+In the absence of Fe the downstream genes are once gain turned on at high cell densities.
 <!-- Add more about the biology of this part here