Difference between revisions of "Part:BBa K3139014"

Revision as of 03:29, 21 October 2019

J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010 anti-plasmodium system

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2232
Illegal NheI site found at 2255
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 444
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2582
Illegal SapI.rc site found at 2930

@@ Line 6: / Line 6: @@
 <!-- Add more about the biology of this part here
-===Usage and Biology===
 In the anti-plasmodium system of NAU-2019, the amount of both TEV protease (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139012">BBa_K3139012</a>) and anti-plasmodium fusion protein (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139013">BBa_K3139013</a>) should be kept at a certain proportion to avoid a waste of cell resources.
 If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease.
 By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model.
+===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>