Difference between revisions of "Part:BBa K3183010"
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<partinfo>BBa_K3183010 short</partinfo>
 
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mClover3 is a green fluorescent protein derivative which has been codon optimized for <i>Lactobacillus reuteri</i> 10023C, and may have uses in other <i>Lactobacillus</i> species.
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mClover3 is a green fluorescent protein derivative which can be used in <i>Lactobacillus reuteri</i> 10023C, and may have uses in other <i>Lactobacillus</i> species.
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<!-- Add more about the biology of this part here>
 
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[[File:BBa_K3183010-F-spectrum.png|thumb|left|430px|'''Figure 6:'''  CD27L Endolysin Killing Assay on <i>E. coli</i> versus <i>Bacillus subtilis</i>; The above graph demonstrates the specificity of the CD27L endolysin, by assaying killing on <i>E. coli</i>, the most common chassis used in synthetic biology applications.]]
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[[File:BBa_K3183010-F-spectrum.png|thumb|left|430px|'''Figure 1:'''  mClover3 spectrum]]
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[[File:T--Oxford--Results-ldh.png|thumb|left|430px|'''Figure 2:'''  mClover3 spectrum]]
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====Fluorescence wavelengths====
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Bajar ''et al'' report the following excitation and emission data for mClover3 - <br>
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*'''Excitation max''' - 506nm
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*'''Emission max''' - 518nm
  
  

Revision as of 03:27, 21 October 2019


mClover3 Fluorescent Protein, Codon Optimized for L. reuteri

mClover3 is a green fluorescent protein derivative which has been codon optimized for Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.


Figure 1: mClover3 spectrum
Figure 2: mClover3 spectrum


Fluorescence wavelengths

Bajar et al report the following excitation and emission data for mClover3 -

  • Excitation max - 506nm
  • Emission max - 518nm


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

This part was characterised in the composite part BBa_K3183101. Data for this part is shown below.