Difference between revisions of "Part:BBa K3170996"
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Tobacco etching virus protease, Part of the protease-protease regulation system | Tobacco etching virus protease, Part of the protease-protease regulation system | ||
| + | <ul> | ||
| + | <li>TEV Protease is a recombinant protease derived from Tobacco Etch Virus (TEV) Nla, which is used to excise the affinity tag of the purified fusion protein. TEV protease has strong site specificity and can recognize the seven amino acid sequence of EXXYXQ(G/S) (Glu-Asn-Leu- Tyr-Phe-Gln-Gly). The most common one is ENLYFQG, and its cleavage site is at Between glutamine and glycine or serine.</li> | ||
| + | </ul> | ||
| + | [[File:T--LZU-CHINA--TEVp.png|600px|thumb|center|]] | ||
| + | <ul> | ||
| + | <li>Using the specificity of TEVp, we cleave the recognition sites of ALKBH (Fig.A) and Casp3 (Fig.B) linked by the target sequence, which are active.</li> | ||
| + | </ul> | ||
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Revision as of 03:20, 21 October 2019
TEVp
Tobacco etching virus protease, Part of the protease-protease regulation system
- TEV Protease is a recombinant protease derived from Tobacco Etch Virus (TEV) Nla, which is used to excise the affinity tag of the purified fusion protein. TEV protease has strong site specificity and can recognize the seven amino acid sequence of EXXYXQ(G/S) (Glu-Asn-Leu- Tyr-Phe-Gln-Gly). The most common one is ENLYFQG, and its cleavage site is at Between glutamine and glycine or serine.
- Using the specificity of TEVp, we cleave the recognition sites of ALKBH (Fig.A) and Casp3 (Fig.B) linked by the target sequence, which are active.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]