Difference between revisions of "Part:BBa K108030:Design"

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===Source===
 
===Source===
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LacI Operator sequence was designed according to reference 1.
 
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TARS sequence was designed according to reference 2.
 
  
 
LacI and T7 terminator was cloned from pET-15b(Novagen).
 
LacI and T7 terminator was cloned from pET-15b(Novagen).
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LVA sequence was designed according to reference 3.
 
  
 
===References===
 
===References===

Revision as of 15:15, 24 October 2008

TARS-LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31


Design Notes

...


Source

LacI and T7 terminator was cloned from pET-15b(Novagen).

References

1. Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 2004, 68(3):474–500.

2. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209.

3. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246.