Difference between revisions of "Part:BBa K3183010:Design"

Latest revision as of 01:25, 21 October 2019

mClover3 Fluorescent Protein, Codon Optimized for L. reuteri

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimization was undertaken to conform to RF10 standards and to match existing codon usage tables for Lactobacillus reuteri.

Source

This part was originally characterized by Bajar et al, 2016 through evolution of GFP derivative Clover2: https://www.nature.com/articles/srep20889

References

1. Bajar, Bryce T., et al. “Improving Brightness and Photostability of Green and Red Fluorescent Proteins for Live Cell Imaging and FRET Reporting.” Scientific Reports, vol. 6, no. 1, Feb. 2016, p. 20889. DOI.org (Crossref), doi:10.1038/srep20889.

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	===References===		===References===
		+	1. Bajar, Bryce T., et al. “Improving Brightness and Photostability of Green and Red Fluorescent Proteins for Live Cell Imaging and FRET Reporting.” Scientific Reports, vol. 6, no. 1, Feb. 2016, p. 20889. DOI.org (Crossref), doi:10.1038/srep20889.