Difference between revisions of "Part:BBa K3142012:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sequence has been optimized for lactic acid bacteria. | |
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| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 01:24, 21 October 2019
Glucose starvation promoter（PT-αcrp）
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 26
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 26
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 26
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 26
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence has been optimized for lactic acid bacteria.
Source
NO
References
[1] Busby, S., and Ebright, R. H. (1999) Transcription Activation by Catabolite Activator 519 Protein (CAP). J. Mol. Biol. 293, 199–213. 520
[2] Savery, N. J., Lloyd, G. S., Kainz, M., Gaal, T., Ross, W., Ebright, R. H., Gourse, R. L., and 521 Busby, S. J. W. (1998) Transcription activation at Class II CRP-dependent promoters: identification of 522 determinants in the C-terminal domain of the RNA polymerase α subunit. EMBO J. 17, 3439–344
[3] Lloyd, G. S., Busby, S. J. W., and Savery, N. J. (1998) Spacing Requirements for 529 interactions between the C-terminal domain of α subunit of Escherichia coli RNA polymerase and the 530 cAMP receptor protein. Biochem. J. 420, 413–420.