Difference between revisions of "Part:BBa K103016"
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===Usage and Biology=== | ===Usage and Biology=== | ||
*It's fusion of E. coli outer membrane protein (OmpA) domain (BBa_K103006) with beta-lactamase subunit omega (BBa_K103012) | *It's fusion of E. coli outer membrane protein (OmpA) domain (BBa_K103006) with beta-lactamase subunit omega (BBa_K103012) | ||
Revision as of 14:05, 24 October 2008
OmpA_linker_omega_linker
Usage and Biology
- It's fusion of E. coli outer membrane protein (OmpA) domain (BBa_K103006) with beta-lactamase subunit omega (BBa_K103012)
- Fusion partners are connected via Gly-Ser-Gly linker
- Part of our 'hunter and prey' selection system
- Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
- Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 802
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 596