Difference between revisions of "Part:BBa K103016"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K103016 short</partinfo> | <partinfo>BBa_K103016 short</partinfo> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | *It's fusion of E. coli outer membrane protein (OmpA) domain (BBa_K103006) with beta-lactamase subunit omega (BBa_K103012) | |
| + | *Fusion partners are connected via Gly-Ser-Gly linker | ||
| + | *Part of our 'hunter and prey' selection system | ||
| + | *Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane | ||
| + | *Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant | ||
| + | *This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:05, 24 October 2008
OmpA_linker_omega_linker
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 802
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 596