Difference between revisions of "Part:BBa K3183008:Design"

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===Design Notes===
 
===Design Notes===
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N/A
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Codon optimized for <i>L. reuteri</i> using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]<br>
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+
RF10, 12, 21, 23, 25, and 1000 Compatible
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===Source===
 
===Source===

Latest revision as of 01:12, 21 October 2019


slpMOD Lactobacillus Secretion Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for L. reuteri using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]
RF10, 12, 21, 23, 25, and 1000 Compatible

Source

The sequence was obtained from Fernandez et al, 2009. doi.org/10.1128/AEM.01728-08 and synthesized by IDT

TBD

References

1. Fernandez, A., et al. “Enhanced Secretion of Biologically Active Murine Interleukin-12 by Lactococcus Lactis.” Applied and Environmental Microbiology, vol. 75, no. 3, Feb. 2009, pp. 869–71. DOI.org (Crossref), doi:10.1128/AEM.01728-08.