Difference between revisions of "Part:BBa K103013"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K103013 short</partinfo> | <partinfo>BBa_K103013 short</partinfo> | ||
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− | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | * beta fragment of the split enzyme TEM beta-lactamase | |
+ | * beta-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] | ||
+ | * works with second half of beta lactamase (part BBa_K103009) | ||
+ | * contains linker module that allows proper binding of fusion partners | ||
+ | * This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 13:42, 24 October 2008
linker fused to omega fragment of TEM beta-lactamase
Usage and Biology
- beta fragment of the split enzyme TEM beta-lactamase
- beta-lactamase activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains [http://www.ncbi.nlm.nih.gov/pubmed/17005637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
- works with second half of beta lactamase (part BBa_K103009)
- contains linker module that allows proper binding of fusion partners
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 190