Difference between revisions of "Part:BBa K3081044"
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This part is an improvement of <partinfo>BBa_K3081024</partinfo>, which we add a degradation signal peptide ssrA to the dCas9. A more gentle decrease in fluorescence is observed when dCas9 is fused to ssrA tag. | This part is an improvement of <partinfo>BBa_K3081024</partinfo>, which we add a degradation signal peptide ssrA to the dCas9. A more gentle decrease in fluorescence is observed when dCas9 is fused to ssrA tag. | ||
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| + | https://2019.igem.org/wiki/images/f/fb/T--Peking--CRISPRi_ssrA_ctrl_NT1.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 01:01, 21 October 2019
pBAD-dCas9-ssrA-J23119-NT1-J23119-mRFP
This part is an improvement of BBa_K3081024, which we add a degradation signal peptide ssrA to the dCas9. A more gentle decrease in fluorescence is observed when dCas9 is fused to ssrA tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 2321
Illegal NheI site found at 5495
Illegal NheI site found at 5518
Illegal NheI site found at 5626
Illegal NheI site found at 5649 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 4600 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 6209
Illegal AgeI site found at 6321 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961