Difference between revisions of "Part:BBa K3081024"
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This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. Fluorescence is decreased as the concentration of arabinose increases. | This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. Fluorescence is decreased as the concentration of arabinose increases. | ||
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+ | https://2019.igem.org/wiki/images/f/fb/T--Peking--CRISPRi_ssrA_ctrl_NT1.png | ||
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Revision as of 01:00, 21 October 2019
pBAD-dCas9-J23119-NT1-J23119-mRFP
This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (NT1) that is complementary to the corresponding sequence of mRFP coding region. Fluorescence is decreased as the concentration of arabinose increases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 5459
Illegal NheI site found at 5482
Illegal NheI site found at 5590
Illegal NheI site found at 5613 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1470
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 6173
Illegal AgeI site found at 6285 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961