Difference between revisions of "Part:BBa K3320013"
 
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===Usage and Biology===
 
===Usage and Biology===
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GFP is a fluorescent protein from Aequorea victoria. By adding the HlyA sequence to the C-terminus of the gene, this allows the fusion of this signal peptide to the GFP, enabling its secretion directly from the cytosol into the extracellular environment.
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GFP is a fluorescent protein from Aequorea victoria. By fusing the HlyA sequence, a peptide consisting of 60 amino acids,  to the C-terminus of the gene of GFP, allows the secretion directly from the cytosol into the extracellular environment by the type 1 secretion system HlyB–HlyD–TolC complex.  
  
  

Latest revision as of 00:52, 21 October 2019


GFPmut3 + HlyA

This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system.

Usage and Biology

GFP is a fluorescent protein from Aequorea victoria. By fusing the HlyA sequence, a peptide consisting of 60 amino acids, to the C-terminus of the gene of GFP, allows the secretion directly from the cytosol into the extracellular environment by the type 1 secretion system HlyB–HlyD–TolC complex.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]