Difference between revisions of "Part:BBa K3001003"

 
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<partinfo>BBa_K3001003 short</partinfo>
 
<partinfo>BBa_K3001003 short</partinfo>
  
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Inducible construct for Cas13a Lbu with purification tags.
+
This protein is an RNA cleaving enzyme called Cas13a that was isolated from <i> Leptotrichia buccalis </i> (Lbu). It interacts with a direct repeat stem loop on a CRISPR RNA (crRNA) that contains a specific sequence for a target RNA transcript. This construct is designed to be able to be expressed under an inducible promoter (T7). The protein itself has several purification tags (His, Twin strep tag, and a SUMO tag). This construct was ordered from Addgene.  
  
 
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Revision as of 00:50, 21 October 2019


Cas13a Lbu + T7 promoter

This protein is an RNA cleaving enzyme called Cas13a that was isolated from Leptotrichia buccalis (Lbu). It interacts with a direct repeat stem loop on a CRISPR RNA (crRNA) that contains a specific sequence for a target RNA transcript. This construct is designed to be able to be expressed under an inducible promoter (T7). The protein itself has several purification tags (His, Twin strep tag, and a SUMO tag). This construct was ordered from Addgene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 107
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 297
    Illegal BglII site found at 845
    Illegal BglII site found at 1781
    Illegal BglII site found at 1991
    Illegal BglII site found at 2045
    Illegal BglII site found at 2276
    Illegal BglII site found at 2549
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1564
    Illegal PstI site found at 2419
    Illegal PstI site found at 3358
    Illegal NgoMIV site found at 2497
    Illegal NgoMIV site found at 3133
  • 1000
    COMPATIBLE WITH RFC[1000]