Difference between revisions of "Part:BBa K3183025:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | The linker is typically assembled onto larger parts during PCR, by including its sequence as an overhang to the appropriate primer. The PCR product can then be further assembled into more complex constructs by Gibson Assembly. | ||
Latest revision as of 00:23, 21 October 2019
Start Methionine-Glycine-Serine Linker Codon-Optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The linker is typically assembled onto larger parts during PCR, by including its sequence as an overhang to the appropriate primer. The PCR product can then be further assembled into more complex constructs by Gibson Assembly.
Source
Made by Oxford 2019