Difference between revisions of "Part:BBa K3183014:Design"
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| + | The linker is typically assembled onto larger parts during PCR, by including its sequence as an overhang to the appropriate primer. The PCR product can then be further assembled into more complex constructs by Gibson Assembly, such that the individual domains are spaced by a hydrophilic linked which allows them to fold independently. | ||
<partinfo>BBa_K3183014 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3183014 SequenceAndFeatures</partinfo> | ||
Latest revision as of 00:12, 21 October 2019
Gly-Ser-Gly Protein Domain Linker for E. coli
The linker is typically assembled onto larger parts during PCR, by including its sequence as an overhang to the appropriate primer. The PCR product can then be further assembled into more complex constructs by Gibson Assembly, such that the individual domains are spaced by a hydrophilic linked which allows them to fold independently.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Compatible to RF10, 12, 21, 23, 25, and 1000
Source
Oxford iGEM Team 2019 - de novo design