Difference between revisions of "Part:BBa K3320015"

Latest revision as of 23:56, 20 October 2019

J23100 + lldR

Second part of the lactate repressor unit from our AND Gate. Constitutive production of Lactate Repressor.

Usage and Biology

This part produces constitutively the LldR, which represses the promoter from BBa_K3320014 in absence of lactate. LldR binds to two operator sites, O1 and O2, with O1 being filled at a lower concentration of LldR. Binding of L-lactate to LldR promotes a conformational change that may disrupt the DNA loop, allowing the formation of the transcription open complex.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 614
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 <!-- Add more about the biology of this part here <!-- -->
 ===Usage and Biology===
-This part produces constitutively the LldR, which represses the promoter from BBa_K3320015 in absence of lactate. LldR binds to two operator sites, O1 and O2, with O1 being filled at a lower concentration of LldR. Binding of L-lactate to LldR promotes a conformational change that may disrupt the DNA loop, allowing the formation of the transcription open complex.
+This part produces constitutively the LldR, which represses the promoter from BBa_K3320014 in absence of lactate. LldR binds to two operator sites, O1 and O2, with O1 being filled at a lower concentration of LldR. Binding of L-lactate to LldR promotes a conformational change that may disrupt the DNA loop, allowing the formation of the transcription open complex.
 <span class='h3bb'>Sequence and Features</span>