Difference between revisions of "Part:BBa K3286205"
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<li>B. R. Pursley, M. M. Maiden, M. L. Hsieh, N. L. Fernandez, G. B. Severin, and C. M. Waters, “Cyclic di-GMP regulates TfoY in Vibrio cholerae to control motility by both transcriptional and posttranscriptional mechanisms,” J. Bacteriol., vol. 200, no. 7, pp. 1–19, 2018.</li>
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<li>S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.</li>
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<!-- Add more about the biology of this part here

Revision as of 23:54, 20 October 2019


Vc2 Riboswitch + sfGFP

This composite part contains the Vc2 riboswitch (BBa_K3286202), which was used in combination with composite part BBa_K3286204. The function of this part is that upon DSF sensing from part BBa_K3286204, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 riboswitch, which will result in sfGFP (BBa_K3286203) translation.


  1. B. R. Pursley, M. M. Maiden, M. L. Hsieh, N. L. Fernandez, G. B. Severin, and C. M. Waters, “Cyclic di-GMP regulates TfoY in Vibrio cholerae to control motility by both transcriptional and posttranscriptional mechanisms,” J. Bacteriol., vol. 200, no. 7, pp. 1–19, 2018.
  2. S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]