Difference between revisions of "Part:BBa K3286205"
Line 3: Line 3:
 
<partinfo>BBa_K3286205 short</partinfo>
 
<partinfo>BBa_K3286205 short</partinfo>
  
−
This  composite part contains the Vc2 riboswitch (<partinfo>BBa_K3286202</partinfo>), which was used in combination with composite part<partinfo>BBa_K3286204</partinfo>. The function of this part is that upon DSF sensing from part <partinfo>BBa_K3286204</partinfo>, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 Riboswitch, which will result in sfGFP (<partinfo>K3286203</partinfo>) translation.
+
This  composite part contains the Vc2 riboswitch (<partinfo>BBa_K3286202</partinfo>), which was used in combination with composite part <partinfo>BBa_K3286204</partinfo>. The function of this part is that upon DSF sensing from part <partinfo>BBa_K3286204</partinfo>, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 riboswitch, which will result in sfGFP (<partinfo>K3286203</partinfo>) translation.
  
  

Revision as of 23:51, 20 October 2019


Vc2 Riboswitch + sfGFP

This composite part contains the Vc2 riboswitch (BBa_K3286202), which was used in combination with composite part BBa_K3286204. The function of this part is that upon DSF sensing from part BBa_K3286204, the levels of c-di-GMP will be decreased in the cell. This results in alleviation of the repression of the Vc2 riboswitch, which will result in sfGFP (BBa_K3286203) translation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]