Difference between revisions of "Part:BBa K3286202"

Revision as of 23:34, 20 October 2019

Vc2 Riboswitch

This is the regulatory sequence of the Vc2 riboswitch. The Vc2 riboswitch is a class I c-di-GMP riboswitch which acts on the translational level. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression and translation of the gene will occur [1,2].

Vc2 riboswitches are regulatory elements naturally occurring in Vibrio cholerea. Although, class I riboswitches in general are phylogenetically widespread among the bacterial kingdom [3].

S. Inuzuka et al., “Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism,” J. Biochem., vol. 160, no. 3, pp. 153–162, 2016.
S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.
N. Sudarsan et al., “Riboswitches in Eubacteria Sense the Second Messenger Cyclic Di-GMP,” Science (80-. )., vol. 321, no. 5887, pp. 411–413, Jul. 2008.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286202 short</partinfo>
-This is the regulatory sequence of the Vc2 riboswitch. The Vc2 riboswitch is a class I c-di-GMP riboswitch. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression and translation of the gene will occur [1,2].
+This is the regulatory sequence of the Vc2 riboswitch. The Vc2 riboswitch is a class I c-di-GMP riboswitch which acts on the translational level. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression and translation of the gene will occur [1,2].
-Vc2 riboswitches are regulatory elements naturally occurring in <i>Vibrio cholerea</i>. But these type of riboswitches are phylogenetically widespread among the bacterial kingdom [3].
+Vc2 riboswitches are regulatory elements naturally occurring in <i>Vibrio cholerea</i>. Although, class I riboswitches in general are phylogenetically widespread among the bacterial kingdom [3].