Difference between revisions of "Part:BBa K3037006"
(→‎Characterization)
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Made by the TU Dresden 2019 adapted to the RFC 25 standard [https://2019.igem.org/Team:TU_Dresden/Parts (more information).]
 
Made by the TU Dresden 2019 adapted to the RFC 25 standard [https://2019.igem.org/Team:TU_Dresden/Parts (more information).]
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== Characterization ==
 
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The Strep-tag [https://parts.igem.org/Part:BBa_K3037009 BBa_K3037009] in this BioBrick was used for make a protein purification
 
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following the protocol “Expression and purification of proteins using Strep-Tactin” of IBA Lifescience using buffers without EDTA.
 
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<gallery mode="packed", caption="Purification of proteins", widths=400px, heights=200px>
 
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File:T--TU_Dresden--Strep-tag_purification_BBa_3037009.png|<span style="color:#0000ff"> ''Strep-tactin column purification''  </span> (Purification of the composite BioBrick BBa_K3037009)
 
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</gallery>
 
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From the results of the purifications we conclude that the Strep-tag doesn't works for column purification, and should be used only for Western Blots as it was meant to be.
 
  
 
== Sequence ==
 
== Sequence ==

Revision as of 22:49, 20 October 2019

eGFP

eGFP
Function Reporter
Use in Escherichia coli
RFC standard Freiburg RFC25 standard
Backbone pSB1C3
Submitted by Team: TU_Dresden 2019



Overview

Made by the TU Dresden 2019 adapted to the RFC 25 standard (more information).

Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]