Difference between revisions of "Part:BBa K3179106:Design"
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===References=== | ===References=== | ||
| − | 1 Wroblewska L, Kitada T, Endo K, et al. Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery[J]. Nature biotechnology, 2015, 33(8): 839. | + | 1 Wroblewska L, Kitada T, Endo K, et al. Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery[J]. Nature biotechnology, 2015, 33(8): 839.<br style="clear: both" /> |
| − | 2 Berk A J. Functions of adenovirus E1A[J]. Cancer surveys, 1986, 5(2): 367-387. | + | 2 Berk A J. Functions of adenovirus E1A[J]. Cancer surveys, 1986, 5(2): 367-387.<br style="clear: both" /> |
| − | 3 Martin M E D, Berk A J. Adenovirus E1B 55K represses p53 activation in vitro[J]. Journal of virology, 1998, 72(4): 3146-3154. | + | 3 Martin M E D, Berk A J. Adenovirus E1B 55K represses p53 activation in vitro[J]. Journal of virology, 1998, 72(4): 3146-3154.<br style="clear: both" /> |
4 Kim J H, Lee S R, Li L H, et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice[J]. PloS one, 2011, 6(4): e18556. | 4 Kim J H, Lee S R, Li L H, et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice[J]. PloS one, 2011, 6(4): e18556. | ||
Latest revision as of 22:27, 20 October 2019
pT2mK2E
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1750
Illegal XbaI site found at 4374
Illegal PstI site found at 2500 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2500
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3326
Illegal BamHI site found at 954
Illegal BamHI site found at 3578 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1750
Illegal XbaI site found at 4374
Illegal PstI site found at 2500 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1750
Illegal XbaI site found at 4374
Illegal PstI site found at 2500
Illegal NgoMIV site found at 1224
Illegal NgoMIV site found at 2219
Illegal NgoMIV site found at 3848
Illegal AgeI site found at 960
Illegal AgeI site found at 3584 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3352
Design Notes
This part consists of miR663b-target,2kt-E1A, E1B 55K and miR885-5p-target, which are put in the vector pTRE. From promoter CMV our plasmid can transcribe mRNA with miR663b-target, 2kt-E1A, E1B 55K and miR885-5p-target, whose translation can be regulated by level of free miRNA663b and miRNA885-5p, and also by L7Ae. When they exist, the translation of this mRNA will be suppressed. The production is E1A and E1B 55K, E1A can start the expression of adenoviral gene, and E1B 55K can enhance the ability of virus to lyse the cell. During assembling, we use P2A sequence link E1A and E1B 55K, which can maintain the expression level of two gene when sharing one promoter.
Source
pTRE-mi663b-2Kt-E1-E1B55K-miR885-5p
References
1 Wroblewska L, Kitada T, Endo K, et al. Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery[J]. Nature biotechnology, 2015, 33(8): 839.
2 Berk A J. Functions of adenovirus E1A[J]. Cancer surveys, 1986, 5(2): 367-387.
3 Martin M E D, Berk A J. Adenovirus E1B 55K represses p53 activation in vitro[J]. Journal of virology, 1998, 72(4): 3146-3154.
4 Kim J H, Lee S R, Li L H, et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice[J]. PloS one, 2011, 6(4): e18556.