Difference between revisions of "Part:BBa K112408"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K112408 short</partinfo>
 
<partinfo>BBa_K112408 short</partinfo>
  
 
[T4 holin][T4 endolysin][dblTerm][T4 antiholin][rnpB T1] in T4 holin
 
[T4 holin][T4 endolysin][dblTerm][T4 antiholin][rnpB T1] in T4 holin
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----
  
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T4 phage derived lysis device with holin, lysozyme and antiholin under a constitutive promoter
  
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This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 04:24, 24 October 2008

T4 phage derived lysis device

[T4 holin][T4 endolysin][dblTerm][T4 antiholin][rnpB T1] in T4 holin

T4 phage derived lysis device with holin, lysozyme and antiholin under a constitutive promoter

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
    Illegal NheI site found at 1370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 67
    Illegal NgoMIV site found at 1700
    Illegal AgeI site found at 953
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1602