Difference between revisions of "Part:BBa K2940014"

Revision as of 22:03, 20 October 2019

XylR coding device-sfGFP_optimism coding device with Pu

This part encodes the biosensor genetic circuit comprising the toluene-specific transcription factor XylR and the sfGFP reporter gene. This part is a modification of the BBa_K2023015 part originally developed by Ionis Paris iGEM team 2016 [1]. The part was meant to serve as the starting biosensor circuit platform for the subsequent directed evolution of XylR in order to develop a biosensor for the detection of aromatic amines.

Usage and Biology

BTEX (Benzene, Toluene, Ethylbenzene, Xylene)-like aromatic compounds detection.

Characterization

The E. coli TOP10 cells transformed with XylR:sfGFP_pSB1C3 biosensor plasmid exhibited strong fluorescence even when uninduced. This strong background fluorescence was most presumably the result of sfGFP leaky expression and made cells apparently not sensitive to the induction when grown on plain and 10 mg/L toluene-containing LB agar plates (Figure 1).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2446
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2101
Illegal XhoI site found at 3018
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2940014 short</partinfo>
-This part encodes the biosensor genetic circuit comprising the toluene-specific transcription factor XylR and the sfGFP reporter gene. The part was meant to serve as the starting biosensor circuit platform for the subsequent directed evolution of XylR in order to develop a biosensor for the detection of aromatic amines.
+This part encodes the biosensor genetic circuit comprising the toluene-specific transcription factor XylR and the sfGFP reporter gene. This part is a modification of the BBa_K2023015 part originally developed by Ionis Paris iGEM team 2016 [1]. The part was meant to serve as the starting biosensor circuit platform for the subsequent directed evolution of XylR in order to develop a biosensor for the detection of aromatic amines.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+BTEX (Benzene, Toluene, Ethylbenzene, Xylene)-like aromatic compounds detection.
+===Characterization===
+The E. coli TOP10 cells transformed with XylR:sfGFP_pSB1C3 biosensor plasmid exhibited strong fluorescence even when uninduced. This strong background fluorescence was most presumably the result of sfGFP leaky expression and made cells apparently not sensitive to the induction when grown on plain and 10 mg/L toluene-containing LB agar plates (Figure 1).
 <!-- -->