Difference between revisions of "Part:BBa K2936017"
 
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<partinfo>BBa_K2936017 short</partinfo>
 
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<img src="https://static.igem.org/mediawiki/parts/8/87/T--ZJUT-China--igem-z5i9.png" width="300px">
 
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pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its expression.
 
pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its expression.
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===Short description===
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A bidirectional switch
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===Description===
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pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its= expression. Those two promoter and the ara operon form a bidirectional switch determined by formaldehyde concentration.
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===Source===
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<i>Escherichia coli.</i >
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===Design consideration===
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There is a leak problem with the FrmR promoter.
  
 
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Latest revision as of 21:48, 20 October 2019


pFrmR-AraC

pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its expression.

Short description

A bidirectional switch

Description

pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The AraC protein would binds to AraBAD promoter, inhibiting its= expression. Those two promoter and the ara operon form a bidirectional switch determined by formaldehyde concentration.

Source

Escherichia coli.

Design consideration

There is a leak problem with the FrmR promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]