Difference between revisions of "Part:BBa K3320013"

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===Usage and Biology===
 
===Usage and Biology===
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GFP is a fluorescent protein from Aequorea victoria. This is a fusion protein with the HlyA secretion tag from the alpha-hemolysin system of E. coli.  It allows the GFP to be secreted directly from the cytosol to the extracellular medium.
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GFP is a fluorescent protein from Aequorea victoria. By adding the HlyA sequence to the C-terminus of the gene, this allows the fusion of this signal peptide to the GFP, enabling its secretion directly from the cytosol into the extracellular environment.
  
  

Revision as of 21:29, 20 October 2019


GFPmut3 + HlyA

This is GFPmut3 based on BBa_E0040 with a secretion tag from the alpha-hemolysin system.

Usage and Biology

GFP is a fluorescent protein from Aequorea victoria. By adding the HlyA sequence to the C-terminus of the gene, this allows the fusion of this signal peptide to the GFP, enabling its secretion directly from the cytosol into the extracellular environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]