Difference between revisions of "Part:BBa K3228075"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228075 short</partinfo>
 
<partinfo>BBa_K3228075 short</partinfo>
  
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test
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===Sequence and Features===
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<partinfo>BBa_K3228075 SequenceAndFeatures</partinfo>
  
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===Description===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3228075 SequenceAndFeatures</partinfo>
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This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range <a href="https://parts.igem.org/Part:BBa_K3228073"> BBa_K3228073</a> to <a href="https://parts.igem.org/Part:BBa_K3228075"> BBa_K3228075</a> as well as <a href="https://parts.igem.org/Part:BBa_K3228090"> BBa_K3228090</a>) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria <a href="https://parts.igem.org/Part:BBa_K3228069"> BBa_K3228069</a> and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder <a href="https://parts.igem.org/Part:BBa_K3228060"> (compare BBa_K3228060)</a> for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization.
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<div style="width:100%;display:flex;flex-direction:row;flex-wrap: wrap; justify-content:space-evenly; align-items:center;">[[File:GE LVL2 measurementvectors.png|500px|thumb|left|'''Figure 2''':  Design of the standardized measurement vectors for terminator BioBrick characterization  ]]
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In our <a href="https://2019.igem.org/Team:Marburg/Results">results</a>we show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility.
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For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for <a href="http://2018.igem.org/Team:Marburg/Results"> Vibrio natriegens in the last year</a>.
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===Compability===
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These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design">Design of the Marburg Collection</a>.
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===Available standard measurement vectors===
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<div class="PCListIcon" style="display:flex;flex-direction:row; flex-wrap: nowrap; justify-content:space-evenly; align-items:flex-start;font-size:82%;">
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[[File:Icon MesurementVector.png|90px|thumb|none|
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<li> <a href="https://parts.igem.org/Part:BBa_K3228073">K3228073 </a> <br>(promoter entry vector ) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228074">K3228074 </a> <br>(rbs entry vector) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228075">K3228075 </a> <br>(CDS entry vector) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228090">K3228090 </a> <br>(terminator entry vector) </li>
  
  
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===Functional Parameters===
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<partinfo>BBa_K3228075 parameters</partinfo>
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Revision as of 21:21, 20 October 2019


pMC_1_003_characterization_CDS_S.e.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2818
    Illegal PstI site found at 5353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 427
    Illegal NheI site found at 450
    Illegal PstI site found at 5353
    Illegal NotI site found at 1620
    Illegal NotI site found at 7357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6516
    Illegal XhoI site found at 2868
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2818
    Illegal PstI site found at 5353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2818
    Illegal PstI site found at 5353
    Illegal NgoMIV site found at 1667
    Illegal NgoMIV site found at 1809
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range BBa_K3228073 to BBa_K3228075 as well as BBa_K3228090) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria BBa_K3228069 and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder (compare BBa_K3228060) for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization.

Figure 2: Design of the standardized measurement vectors for terminator BioBrick characterization

In our resultswe show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility. For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for Vibrio natriegens in the last year.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Available standard measurement vectors