Difference between revisions of "Part:BBa K112402:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K112402 short</partinfo>
 
<partinfo>BBa_K112402 short</partinfo>
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===Design Notes===
 
===Design Notes===
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none
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==pBca1256-K112402==
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<pre>
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Biobricking of promoter for fabA protein
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PCR fabA-F and fabA-R on MG1655   (285 bp, EcoR1/BamH1)
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Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
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Product is pBca1256-K112402
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----------------------------
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cb0005-fabA-F Forward Biobricking  cgataGAATTCatgAGATCTggccattacgttggctgaac
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cb0006-fabA-R Reverse Biobricking  cgtagGGATCCgaagttaccacccgtttcgg
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</pre>
  
  

Latest revision as of 04:09, 24 October 2008

promoter for FabA gene - Membrane Damage and Ultrasound Senstitive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pBca1256-K112402

Biobricking of promoter for fabA protein 
 PCR fabA-F and fabA-R on MG1655 	  (285 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112402
 ----------------------------
cb0005-fabA-F Forward Biobricking   cgataGAATTCatgAGATCTggccattacgttggctgaac 
cb0006-fabA-R Reverse Biobricking   cgtagGGATCCgaagttaccacccgtttcgg


Source

cloned from E. coli MG1655 genome

References