Difference between revisions of "Part:BBa K112401:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K112401 short</partinfo>
 
<partinfo>BBa_K112401 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We did not see the desired response from ultrasound exposure that we expected from this promoter. However, we did not induce the same acoustic cavitation that Vollmer et. al. used to elicit a response from this promoter.
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==pBca1256-K112401==
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<pre>
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Biobricking of promoter for recA protein
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PCR recA-F and recA-R on MG1655   (317 bp, EcoR1/BamH1)
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Sub into pBca1256                   (EcoR1/BamH1 910+2472bp, L)
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Product is pBca1256-K112401
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----------------------------
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cb0003-recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
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cb0004-recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc
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</pre>
  
  

Latest revision as of 04:08, 24 October 2008

Promoter for recA gene - SOS and Ultrasound Sensitive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pBca1256-K112401

Biobricking of promoter for recA protein 
 PCR recA-F and recA-R on MG1655 	  (317 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
 ----------------------------
cb0003-recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
cb0004-recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc


Source

Cloned from E. coli MG1655 genome.

References