Difference between revisions of "Part:BBa K112400:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K112400 short</partinfo>
 
<partinfo>BBa_K112400 short</partinfo>
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===Design Notes===
 
===Design Notes===
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none
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==pBca1256-K112400==
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<pre>
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Biobricking of promoter for grpE protein
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PCR grpE-F and grpE-R on MG1655   (129 bp, EcoRI/BamHI)
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Sub into pBca1256                   (EcoRI/BamHI 910+2472bp, L)
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Product is pBca1256-K112400
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----------------------------
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cb0001-grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
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cb0002-grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc
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</pre>
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Latest revision as of 04:08, 24 October 2008

Promoter for grpE gene - Heat Shock and Ultrasound Sensitive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pBca1256-K112400

Biobricking of promoter for grpE protein 
 PCR grpE-F and grpE-R on MG1655 	  (129 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
cb0001-grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
cb0002-grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc



Source

cloned from E. coli MG1655 genome

References