Difference between revisions of "Part:BBa K3016100"

Revision as of 20:38, 20 October 2019

Vibrio natriegens' TorA signal peptide

This part contains Vibrio natriegens' TorA Tat signal peptide. It is a twin-arginine (RR) motif containing signal peptide for periplasmic transport of proteins via the twin-arginine translocation (Tat) pathway. Derived from Vibrio natriegens’ torA gene.

Biology

The twin-arginine translocation (Tat) pathway is capable of translocating fully folded proteins up to 150 kDa. It also contains a quality control feature of rejecting misfolded proteins. In some cases, disulfide bridge formation is not required for successful translocation. (Alanen et al., 2015)

Translocation using the tat-pathway requires the protein to contain a N-terminal signal peptide with a twin-arginine (RR) motif. The signal peptide is cleaved during the translocation process. A pair of V. natriegens’ native twin-arginine signal peptides identified by Aalto-Helsinki can be found here (TorAand Aminotransferase)

Use

This part can be N-terminally fused with your protein of choice to facilitate its periplasmic transport via the twin-arginine translocation (Tat) pathway.

Aalto-Helsinki 2019 used this part by combining it with YGFP, a slow-bleaching GFP variant, creating a composite TorA-YGFP part ([ ]) to test protein translocation into Vibrio natriegens' and Escherichia coli's periplasm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3016100 short</partinfo>
-*edit*
+This part contains <i>Vibrio natriegens'</i> TorA Tat signal peptide. It is a twin-arginine (RR) motif containing signal peptide for periplasmic transport of proteins via the twin-arginine translocation (Tat) pathway. Derived from Vibrio natriegens’ <i>torA gene</i>.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+==Biology==
+The twin-arginine translocation (Tat) pathway is capable of translocating fully folded proteins up to 150 kDa. It also contains a quality control feature of rejecting misfolded proteins. In some cases, disulfide bridge formation is not required for successful translocation. (Alanen et al., 2015)
+Translocation using the tat-pathway requires the protein to contain a N-terminal signal peptide with a twin-arginine (RR) motif. The signal peptide is cleaved during the translocation process. A pair of <i>V. natriegens’</i> native twin-arginine signal peptides identified by Aalto-Helsinki can be found here ([https://parts.igem.org/Part:BBa_K3016100 TorA]and [https://parts.igem.org/Part:BBa_K3016100 Aminotransferase])
+===Use===
+This part can be N-terminally fused with your protein of choice to facilitate its periplasmic transport via the twin-arginine translocation (Tat) pathway.
+Aalto-Helsinki 2019 used this part by combining it with YGFP, a slow-bleaching GFP variant, creating a composite TorA-YGFP part ([ ]) to test protein translocation into <i>Vibrio natriegens'</i> and <i>Escherichia coli's</i> periplasm.
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