Difference between revisions of "Part:BBa K3292005"

Revision as of 20:35, 20 October 2019

DNA synthesis regulated promoter (yorB)

This promoter regulates the expression of the YorB protein in B. subtilis subtilis str. 168 and was initally reported by Hutter et al. (2004) and further characterized by Urban et al. (2007) for its induction by antibiotics that interference with DNA biosynthesis in B. subtilis.

As part of our project, we flanked the core sequence with sites of type IIs restriction enzymes, particularly BsaI and BsmBI (also called Esp3I), to allow its usage with Golden Gate cloning. Finally, we added biobrick prefix and suffix to make it compatible with RFC 10 standard, and M13 forward and reverse primer sequences to enable the PCR amplification of this parts as gBlocks.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 120
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3292005 short</partinfo>
-This promoter regulates the expression of the YorB protein in B. subtilis subtilis str. 168 and was initally reported by Hutter et al. (2004) and further characterized by Urban et al. (2007) for its induction by the antibiotic interference of DNA biosynthesis in B. subtilis.
+This promoter regulates the expression of the YorB protein in B. subtilis subtilis str. 168 and was initally reported by Hutter et al. (2004) and further characterized by Urban et al. (2007) for its induction by antibiotics that interference with DNA biosynthesis in B. subtilis.
-As part of our project, we flanked the core sequence with sites of type IIs restriction enzymes, particularly BsaI and BsmBI (also called  for its isoschizomer's name Esp3I), to allow its usage with Golden Gate cloning. Finally, we added Biobrick prefix and suffix to make it compatible with RFC 10 standard, and M13 forward and reverse primer sequences to enable the PCR amplification of this parts as gBlocks.
+As part of our project, we flanked the core sequence with sites of type IIs restriction enzymes, particularly BsaI and BsmBI (also called Esp3I), to allow its usage with Golden Gate cloning. Finally, we added biobrick prefix and suffix to make it compatible with RFC 10 standard, and M13 forward and reverse primer sequences to enable the PCR amplification of this parts as gBlocks.