Difference between revisions of "Part:BBa K2940003"

Revision as of 20:29, 20 October 2019

Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system

Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.

Usage and Biology

• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production.

• Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.

• Flanking glutamic blocks for solubilization

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 128
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 20
Illegal SpeI site found at 128
Illegal NotI site found at 27
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 128
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 128
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-•	N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production
+• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production.
-•	Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.
-•	Flanking glutamic blocks for solubilization
+• Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.
+• Flanking glutamic blocks for solubilization