Difference between revisions of "Part:BBa K3228057"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228057 short</partinfo>
 
<partinfo>BBa_K3228057 short</partinfo>
  
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test
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===Sequence and Features===
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<partinfo>BBa_K3228057 SequenceAndFeatures</partinfo>
  
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<!-- Add more about the biology of this part here
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===Description===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3228057 SequenceAndFeatures</partinfo>
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This part contains the ribosomal binding site <a href="https://parts.igem.org/Part:BBa_B0034"> B0034</a> and PlmJ which is a ribozyme.
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<br>
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Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).
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<div style="width:100%;display:flex;flex-direction:row;flex-wrap: wrap; justify-content:space-evenly; align-items:center;">[[File:GE riboinsulators.png|500px|thumb|left|'''Figure 1''': Maturation of mRNA transcripts leads to a more standardized genetic context.  ]]
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This leads to a more stable expression independent of the cellular context <a href="http://2016.igem.org/Team:William_and_Mary/RiboJ">(See iGEM William and Mary 2016)</a> and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.
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===Compability===
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These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
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===Available Ribo-insulators===
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<div class="PCListIcon" style="display:flex;flex-direction:row; flex-wrap: nowrap; justify-content:space-evenly; align-items:flex-start;font-size:82%;">
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[[File:Icon Ribo Isulator.png|90px|thumb|none|
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<li> <a href="https://parts.igem.org/Part:BBa_K3228054">K3228054 </a> (riboJ B0034) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228055">K3228055 </a> (riboJ B0034 <br> no Scar) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228056">K3228056 </a> <br> (SarJ B0034) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228057">K3228057 </a> (PlmJ B0034) </li>
  
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===Functional Parameters===
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<partinfo>BBa_K3228057 parameters</partinfo>
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Revision as of 20:18, 20 October 2019


pMC_0_3_15_PlmJ_B0034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part contains the ribosomal binding site B0034 and PlmJ which is a ribozyme.

Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).

Figure 1: Maturation of mRNA transcripts leads to a more standardized genetic context.

This leads to a more stable expression independent of the cellular context (See iGEM William and Mary 2016) and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Available Ribo-insulators