Difference between revisions of "Part:BBa K3037006"

Revision as of 20:15, 20 October 2019

eGFP

eGFP
Function	Reporter
Use in	Escherichia coli
RFC standard	Freiburg RFC25 standard
Backbone	pSB1C3
Submitted by	Team: TU_Dresden 2019

Overview

Made by the TU Dresden 2019 adapted to the RFC 25 standard (more information).

Characterization

The Strep-tag BBa_K3037009 in this BioBrick was used for make a protein purification following the protocol “Expression and purification of proteins using Strep-Tactin” of IBA Lifescience using buffers without EDTA.

Strep-tactin column purification (Purification of the composite BioBrick BBa_K3037009)

From the results of the purifications we conclude that the Strep-tag doesn't works for column purification, and should be used only for Western Blots as it was meant to be.

Sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 27: / Line 27: @@
 Made by the TU Dresden 2019 adapted to the RFC 25 standard [https://2019.igem.org/Team:TU_Dresden/Parts (more information).]
+== Characterization ==
+The Strep-tag [https://parts.igem.org/Part:BBa_K3037009 BBa_K3037009] in this BioBrick was used for make a protein purification
+following the protocol “Expression and purification of proteins using Strep-Tactin” of IBA Lifescience using buffers without EDTA.
+<gallery mode="packed", caption="Purification of proteins", widths=400px, heights=200px>
+File:T--TU_Dresden--Strep-tag_purification_BBa_3037009.png|<span style="color:#0000ff"> ''Strep-tactin column purification''  </span> (Purification of the composite BioBrick BBa_K3037009)
+</gallery>
+From the results of the purifications we conclude that the Strep-tag doesn't works for column purification, and should be used only for Western Blots as it was meant to be.
 == Sequence ==