Difference between revisions of "Part:BBa K3228043"

 
Line 1: Line 1:
 +
<html>
 +
<div class="titleWrapper"><div class="titleBackground" style="background-image:none"></div><div class="title"></div></div>
 +
<article>
 +
<style>
 +
.imageContainer1x2 {
 +
display: flex;
 +
flex-wrap: wrap;
 +
justify-content: space-around;
 +
align-items: center;
 +
}
 +
.imageContainer1x2 div {
 +
width: 50%;
 +
margin-bottom: 2%;
 +
text-align:center;
 +
}
 +
.imageContainer1x2 div img {
 +
width: 100%;
 +
}
 +
.imageContainer1x4 {
 +
display: flex;
 +
flex-wrap: wrap;
 +
justify-content: space-around;
 +
align-items: center;
 +
}
 +
.imageContainer1x4 div {
 +
width: 23%;
 +
margin-bottom: 2%;
 +
text-align:center;
 +
}
 +
.imageContainer1x4 div img {
 +
width: 100%;
 +
}
 +
<i>
 +
</style>
 +
</html>
  
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228043 short</partinfo>
 
<partinfo>BBa_K3228043 short</partinfo>
  
−
test
+
===Sequence and Features===
 +
<partinfo>BBa_K3228043 SequenceAndFeatures</partinfo>
  
−
<!-- Add more about the biology of this part here
+
===Description===
−
===Usage and Biology===
+
  
−
<!-- -->
+
<html>
−
<span class='h3bb'>Sequence and Features</span>
+
<p align="justify">
−
<partinfo>BBa_K3228043 SequenceAndFeatures</partinfo>
+
specific text here
 +
 
 +
<br>
 +
<br>
 +
This part is contained in a collection of reporters for <i>Synechococcus elongatus</i> UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a> They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on
 +
<a href="https://2019.igem.org/Team:Marburg/Results">experimental data</a>and literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.
 +
 +
</p>
 +
</html>
 +
 
 +
===Compability===
 +
 
 +
<html>
 +
<p align="justify">
 +
 
 +
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
 +
</p>
 +
</html>
 +
 
 +
===Available reporters===
 +
 
 +
<div class="PCListIcon" style="display:flex;flex-direction:row; flex-wrap: nowrap; justify-content:space-evenly; align-items:flex-start;font-size:82%;">
 +
 
 +
[[File:Icon L-Reporter.png|90px|thumb|none|
 +
 
 +
<html>
 +
<ul>
 +
<b>
 +
 
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228040">K3228040 </a> <br>(NanoLuc) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228041">K3228041 </a> <br>(NanoLuc S.e.)</li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228042">K3228042 </a> <br> (teLuc S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228043">K3228043 </a> <br> (Antares S.e.) </li>
 +
</b>
 +
</ul>
 +
</html>]]
  
 +
[[File:Icon F-Reporter.png|90px|thumb|none|
 +
<html>
 +
<ul>
 +
<b>
  
−
<!-- Uncomment this to enable Functional Parameter display
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228044">K3228044 </a> <br> (eYFP S.e.) </li>
−
===Functional Parameters===
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228045">K3228045 </a> <br> (phluorin2 S.e.) </li>
−
<partinfo>BBa_K3228043 parameters</partinfo>
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228046">K3228046 </a> <br> (rxYFP S.e.) </li>
−
<!-- -->
+
<li> <a href="https://parts.igem.org/Part:BBa_K3228047">K3228047 </a> <br> (sfGFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228048">K3228048 </a> <br> (sfGFP S.e.) </li>
 +
<li> <a href="https://parts.igem.org/Part:BBa_K3228049">K3228049 </a> <br> (mTurqoise S.e.) </li>
 +
</b>
 +
</ul>
 +
</html>]]
 +
</div>

Revision as of 20:03, 20 October 2019


pMC_0_4_25_Antares2 (S.e.)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1513
    Illegal PstI site found at 1409
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1513
    Illegal PstI site found at 1409
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1513
    Illegal PstI site found at 1409
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1513
    Illegal PstI site found at 1409
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

specific text here

This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. They are specifically chosen for cyanobacteria as they show a different absorption and excitation behaviour compared to other bacterial cells due to their photosystems. Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set should be seen as a recommendation of reporters based on experimental dataand literature. They are either fluorescence reporters that bypass the wavelength at which cyanobacteria absorb and emit light or luminescent reporters that show a greater signal strength over background signal.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Available reporters