Difference between revisions of "Part:BBa K3286202"

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Vc2 Riboswitch

This is the regulatory sequence of the Vc2 Riboswitch. The Vc2 Riboswitch is a class I c-di-GMP riboswitch. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression, and translation of the gene will occur [1,2].

Vc2 Riboswitches are regulatory elements naturally occurring in Vibrio cholerea. But these type of class I riboswtiches are present in multiple different bacterial species [3].

1. S. Inuzuka et al., “Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism,” J. Biochem., vol. 160, no. 3, pp. 153–162, 2016.
2. S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.
3. N. Sudarsan et al., “Riboswitches in Eubacteria Sense the Second Messenger Cyclic Di-GMP,” Science (80-. )., vol. 321, no. 5887, pp. 411–413, Jul. 2008.

Sequence and Features