Difference between revisions of "Part:BBa K2983050"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | TEF is an isolated promoteur with highly expressed genes in ''yarrowia lipolytica'' and present in only on copy in his genome. TEF encode for translation elongation factor-1 α [1]and is also widely used to drive constitutive gene expression in Y. lipolytica.[2] | + | TEF is an isolated promoteur with highly expressed genes in ''yarrowia lipolytica'' and present in only on copy in his genome. TEF encode for translation elongation factor-1 α [1] and his promoter is also widely used to drive constitutive gene expression in Y. lipolytica.[2] |
===References=== | ===References=== | ||
Revision as of 19:48, 20 October 2019
pTef1c: BBa_K2117000 without the BsaI site
This part is a version of the Yarrowia lipolytica pTef1 promoter. It is an improvement of BBa_K2117000 as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
Usage and Biology
TEF is an isolated promoteur with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α [1] and his promoter is also widely used to drive constitutive gene expression in Y. lipolytica.[2]
References
[1] Müller S, Sandal T, Kamp-Hansen P, Dalbøge H. Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast. 1998 Oct;14(14):1267-83. PMID: 9802206 [2]Trassaert, M. et al. New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica. Microb Cell Fact 16, 141 (2017).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]