Difference between revisions of "Part:BBa K3081057"
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<partinfo>BBa_K3081057 short</partinfo>
 
<partinfo>BBa_K3081057 short</partinfo>
  
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dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.This part is an improvement of<partinfo>BBa_K3081040</partinfo>, which we add a degradation signal peptide ssrA to the dCas9.  
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the bottom strand of initiation site on p15A origin. In this part, we add a degradation signal peptide "ssrA" to the dCas9 to alleviate its effect.  
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 19:24, 20 October 2019

CRISPR-based replication interference system for p15A plasmid copy number control, ini(+) site

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the bottom strand of initiation site on p15A origin. In this part, we add a degradation signal peptide "ssrA" to the dCas9 to alleviate its effect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961