Difference between revisions of "Part:BBa K137120"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K137120 short</partinfo> | <partinfo>BBa_K137120 short</partinfo> | ||
| − | Constitutively expresses the lamB gene, which codes for the lambda receptor. Following the lamB gene is the tetracycline resistance cassette. | + | Constitutively expresses the lamB gene, which codes for the lambda receptor. Following the lamB gene is the tetracycline resistance cassette. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The series of parts K137120-K137123 were placed in plasmid pSB1A2 and ''E. coli'' strain JW3996-1 (''lamB''::''kanR''). When infected with phage lambda, the cells produced the following relative plating efficiency (pfu/mL on engineered strain relative to pfu/mL on wild-type DH10B): | ||
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| + | [[Image:LamBTetA.gif|500px]] | ||
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| + | The plating efficiency correlates to expression level (determined using the promoter and RBS strengths listed in the Registry) with almost perfect linearity, suggesting that LamB expression is limiting infection frequency. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K137120 SequenceAndFeatures</partinfo> | <partinfo>BBa_K137120 SequenceAndFeatures</partinfo> | ||
Latest revision as of 21:14, 23 October 2008
LamB and TetA expression plasmid
Constitutively expresses the lamB gene, which codes for the lambda receptor. Following the lamB gene is the tetracycline resistance cassette.
Usage and Biology
The series of parts K137120-K137123 were placed in plasmid pSB1A2 and E. coli strain JW3996-1 (lamB::kanR). When infected with phage lambda, the cells produced the following relative plating efficiency (pfu/mL on engineered strain relative to pfu/mL on wild-type DH10B):
The plating efficiency correlates to expression level (determined using the promoter and RBS strengths listed in the Registry) with almost perfect linearity, suggesting that LamB expression is limiting infection frequency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 1274 - 1000COMPATIBLE WITH RFC[1000]